A Spectrophotometric Method for the Determination of Reduced Glutathione

A spectrophotometric method for the determination of reduced glutathione.

In a previous communication (1) it was reported that the products formed by the interaction of fluoropyruvic acid with mercaptans exhibit characteristic ultraviolet absorption spectra. CXor /I-aminothiols with an unsubstituted amino group (cysteine, etc.) gave products of high absorbancy, with a peak in wave length between 295 and 300 rnp (Type II spectrum). Those lacking the free amino group (...

A simple spectrophotometric method for the determination of blood sugar by phenylhydrazine Hydrochlorlde

A Highly Sensitive Kinetic Spectrophotometric Method for the Determination of Ascorbic Acid in Pharmaceutical Samples

In this study, a new reaction system for quantitative determination of ascorbic acid was introduced. The developed method is based on inhibitory effect of ascorbic acid on the Orange G-bromate system. The change in absorbance was followed spectrophotometrically at 478 nm. The dependence of sensitivity on the reaction variables including reagents concentration, temperature and time was investiga...

A Highly Sensitive Kinetic Spectrophotometric Method for the Determination of Ascorbic Acid in Pharmaceutical Samples

In this study, a new reaction system for quantitative determination of ascorbic acid was introduced. The developed method is based on inhibitory effect of ascorbic acid on the Orange G-bromate system. The change in absorbance was followed spectrophotometrically at 478 nm. The dependence of sensitivity on the reaction variables including reagents concentration, temperature and time was investiga...

Kinetic Spectrophotometric Method for Determination of S032- Using Methylen blue

The present work reports a kinetic spectrophotometric method for the determination of traceamounts of sulphite that is based on inhibitory effect of sulphite on the oxidation of Methyleneblue (MB) in acidic media. The reaction is followed spectrophotometrically by monitoring thedecrease in absorbance of MB at 664 nm with a fixed time of 0.5-4.5 minutes. It was found thatsulphite exert a strong ...